mouse par1 elisa kit (MyBiosource Biotechnology)

MyBiosource Biotechnology mouse par1 elisa kit

Primers used for the semi-quantification of mRNA levels of coagulation and inflammatory factors in cultured Colon26 cells

Mouse Par1 Elisa Kit, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+par+1/pmc09825203-77-75-80?v=MyBiosource+Biotechnology
Average 90 stars, based on 1 article reviews

mouse par1 elisa kit - by Bioz Stars, 2026-08

90/100 stars

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rabbit anti-mouse igg antibody (Bioss)

Bioss rabbit anti-mouse igg antibody

Rabbit Anti Mouse Igg Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+par+1/custom%40bs-0296r%4040755796?v=Bioss
Average 95 stars, based on 1 article reviews

rabbit anti-mouse igg antibody - by Bioz Stars, 2026-08

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anti-human par1 (f2r) (extracellular) antibody (Alomone Labs)

Alomone Labs anti-human par1 (f2r) (extracellular) antibody

Anti Human Par1 (F2r) (Extracellular) Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+par+1/alomone+labs___apr-031?v=Alomone+Labs
Average 90 stars, based on 1 article reviews

anti-human par1 (f2r) (extracellular) antibody - by Bioz Stars, 2026-08

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rabbit anti-human par-1 / mark2 (internal) (RayBiotech)

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PAR-1 / MARK2 Rabbit anti-Human Polyclonal (Internal) (Unconjugated) Antibody, (50 µg)

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rabbit anti-human par-1 / mark2 (n-terminus) (RayBiotech)

N/A

PAR-1 / MARK2 Rabbit anti-Human Polyclonal (N-Terminus) (Unconjugated) Antibody, (50 µg)

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Image Search Results

Journal: TH Open: Companion Journal to Thrombosis and Haemostasis

Article Title: Edoxaban, a Factor Xa-Specific Direct Oral Anticoagulant, Significantly Suppresses Tumor Growth in Colorectal Cancer Colon26-Inoculated BALB/c Mice

doi: 10.1055/s-0042-1758855

Figure Lengend Snippet: Primers used for the semi-quantification of mRNA levels of coagulation and inflammatory factors in cultured Colon26 cells

Article Snippet: The concentrations of IL-6, matrix metalloproteinase-2 (MMP-2), TF, and PAI-1 in heparinized plasma and those of PAR1, PAR2, STAT3, cyclin D1, Ki67, p53, VEGF-A, and VEGFR-1 in normal or tumor tissues were determined by enzyme-linked immunosorbent assay (ELISA) using mouse IL-6 quantikine ELISA kit (M6000B; R&D Systems, Minneapolis, Minnesota, United States), mouse MMP-2 ELISA kit (ab254516; Abcam, Cambridge, UK), mouse TF ELISA kit (SEA524Ra; Cloud-Clone, Houston, Texas, United States), mouse PAI-1 ELISA kit (ab197752; Abcam), mouse PAR1 ELISA kit (MBS753326; MyBioSource, San Diego, California, United States), mouse PAR2 ELISA kit (MBS4501658; MyBioSource), mouse STAT3 ELISA kit (OKEH02995; Aviva System Biology, San Diego, California, United States), cyclin D1 ELISA kit (NOV596744; Novus, Centennial, California, United States), mouse Ki67 ELISA kit (EM1473; Wuhan Fine Biotech, Hubei, China), mouse p53 ELISA kit (ab224878; Abcam), mouse VEGF-A ELISA kit (BMS619–2; Thermo Fisher Scientific, Waltham, Massachusetts, United States), and mouse VEGFR-1 ELISA kit (CSB-E04762m; CUSABIO, Houston, Texas, United States) respectively, according to the manufacturer's instructions.

Techniques: Coagulation, Cell Culture

( A ) mRNA expression of coagulation and inflammation factor genes in cultured Colon26 cells determined using PCR. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL-6, interleukin-6; MW, molecular weight markers; PAI-1, plasminogen activator inhibitor-1; PAR1, protease-activated receptor-1; PAR2, protease-activated receptor-2;; TF, tissue factor. ( B ) Western blot image of TF protein in total protein 0.25 to 2.5 μg/lane of a cell extract of cultured Colon26 cells. The data indicate that the molecular weight of TF is approximately 48 kDa.

Journal: TH Open: Companion Journal to Thrombosis and Haemostasis

Article Title: Edoxaban, a Factor Xa-Specific Direct Oral Anticoagulant, Significantly Suppresses Tumor Growth in Colorectal Cancer Colon26-Inoculated BALB/c Mice

doi: 10.1055/s-0042-1758855

Figure Lengend Snippet: ( A ) mRNA expression of coagulation and inflammation factor genes in cultured Colon26 cells determined using PCR. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL-6, interleukin-6; MW, molecular weight markers; PAI-1, plasminogen activator inhibitor-1; PAR1, protease-activated receptor-1; PAR2, protease-activated receptor-2;; TF, tissue factor. ( B ) Western blot image of TF protein in total protein 0.25 to 2.5 μg/lane of a cell extract of cultured Colon26 cells. The data indicate that the molecular weight of TF is approximately 48 kDa.

Techniques: Expressing, Coagulation, Cell Culture, Molecular Weight, Western Blot

Tissue levels of coagulation- and tumor-related factors in untreated (UTR) mice and Colon26-inoculated mice orally treated with water or edoxaban (EDX) daily for 21 d

Journal: TH Open: Companion Journal to Thrombosis and Haemostasis

Article Title: Edoxaban, a Factor Xa-Specific Direct Oral Anticoagulant, Significantly Suppresses Tumor Growth in Colorectal Cancer Colon26-Inoculated BALB/c Mice

doi: 10.1055/s-0042-1758855

Figure Lengend Snippet: Tissue levels of coagulation- and tumor-related factors in untreated (UTR) mice and Colon26-inoculated mice orally treated with water or edoxaban (EDX) daily for 21 d

Techniques: Coagulation

( A ) Immunofluorescence data for PAR1 and PAR2 expression in the normal thigh tissues of untreated (UTR) mice and tumor tissues of the water-treated group or edoxaban (EDX) (10 mg/kg/d)-treated group of Colon26-inoculated mice on day 21. ( B ) Immunofluorescence data for STAT3, cyclin D1, and Ki67 expression in normal thigh tissues of untreated (UTR) mice and tumor tissues of the water-treated group or EDX-treated group of Colon26-inoculated mice on day 21.

Journal: TH Open: Companion Journal to Thrombosis and Haemostasis

Article Title: Edoxaban, a Factor Xa-Specific Direct Oral Anticoagulant, Significantly Suppresses Tumor Growth in Colorectal Cancer Colon26-Inoculated BALB/c Mice

doi: 10.1055/s-0042-1758855

Figure Lengend Snippet: ( A ) Immunofluorescence data for PAR1 and PAR2 expression in the normal thigh tissues of untreated (UTR) mice and tumor tissues of the water-treated group or edoxaban (EDX) (10 mg/kg/d)-treated group of Colon26-inoculated mice on day 21. ( B ) Immunofluorescence data for STAT3, cyclin D1, and Ki67 expression in normal thigh tissues of untreated (UTR) mice and tumor tissues of the water-treated group or EDX-treated group of Colon26-inoculated mice on day 21.

Techniques: Immunofluorescence, Expressing

( A ) Images of immunofluorescence analysis of TUNEL-positive cells and control DAPI staining in normal thigh tissue of untreated (UTR) mice and tumor tissue in the water-treated group or edoxaban (EDX) (10 mg/kg/d)-treated group of Colon26-inoculated mice on day 21. ( B ) TUNEL-positive cells/mm 2 in each group of tissues shown in Fig. 5A were counted as described in the text. The data are shown as mean ± SD. Significant differences in TUNEL-positive cells between groups are shown as ** p <0.01. ( C ) The concentration of p53 protein, determined by ELISA, in solubilized samples (100 mg) of normal thigh tissue from untreated (UTR) mice and tumor tissues from the water-treated group or EDX-treated group of Colon26-inoculated mice on day 21. The data are shown as mean ± SD. Significant differences in p53 protein concentrations between groups are shown as ** p <0.01.

Journal: TH Open: Companion Journal to Thrombosis and Haemostasis

Article Title: Edoxaban, a Factor Xa-Specific Direct Oral Anticoagulant, Significantly Suppresses Tumor Growth in Colorectal Cancer Colon26-Inoculated BALB/c Mice

doi: 10.1055/s-0042-1758855

Figure Lengend Snippet: ( A ) Images of immunofluorescence analysis of TUNEL-positive cells and control DAPI staining in normal thigh tissue of untreated (UTR) mice and tumor tissue in the water-treated group or edoxaban (EDX) (10 mg/kg/d)-treated group of Colon26-inoculated mice on day 21. ( B ) TUNEL-positive cells/mm 2 in each group of tissues shown in Fig. 5A were counted as described in the text. The data are shown as mean ± SD. Significant differences in TUNEL-positive cells between groups are shown as ** p <0.01. ( C ) The concentration of p53 protein, determined by ELISA, in solubilized samples (100 mg) of normal thigh tissue from untreated (UTR) mice and tumor tissues from the water-treated group or EDX-treated group of Colon26-inoculated mice on day 21. The data are shown as mean ± SD. Significant differences in p53 protein concentrations between groups are shown as ** p <0.01.

Techniques: Immunofluorescence, TUNEL Assay, Control, Staining, Concentration Assay, Enzyme-linked Immunosorbent Assay

( A ) Concentrations of VEGF-A and ( B ) VEGFR-1, determined by ELISA, in solubilized samples (100 mg) of normal thigh tissue from untreated (UTR) mice and tumor tissues from water-treated group or EDX-treated group of Colon26-inoculated mice on day 21. The data are shown as mean ± SD. Significant differences in concentrations of the respective proteins between groups are shown as ** p <0.01.

Journal: TH Open: Companion Journal to Thrombosis and Haemostasis

Article Title: Edoxaban, a Factor Xa-Specific Direct Oral Anticoagulant, Significantly Suppresses Tumor Growth in Colorectal Cancer Colon26-Inoculated BALB/c Mice

doi: 10.1055/s-0042-1758855

Figure Lengend Snippet: ( A ) Concentrations of VEGF-A and ( B ) VEGFR-1, determined by ELISA, in solubilized samples (100 mg) of normal thigh tissue from untreated (UTR) mice and tumor tissues from water-treated group or EDX-treated group of Colon26-inoculated mice on day 21. The data are shown as mean ± SD. Significant differences in concentrations of the respective proteins between groups are shown as ** p <0.01.

Techniques: Enzyme-linked Immunosorbent Assay

Effect of edoxaban (EDX) on plasma thrombin-antithrombin complex (TAT) levels in untreated (UTR) control mice and Colon26-inoculated mice. Seven days (day 0) after inoculating mice with Colon26 cells, water or EDX (10 mg/kg/d) was orally administered daily for 21 days; citrated plasma samples were prepared from blood drawn from the heart on days -7, 0, 7, 14, and 21 of UTR mice ( n = 5) (○), water-treated group ( n = 5) (●), or EDX-treated group ( n = 5) (▪) of Colon26-inoculated mice. Plasma TAT concentration was determined by ELISA. Significant differences in plasma TAT levels between the water-treated group or EDX-treated group of Colon26-inoculated mice and the UTR mouse group are shown as ** p <0.01. Significant differences in plasma TAT levels between the water-treated group and EDX-treated group of Colon26-inoculated mice are shown as † p <0.05.

Journal: TH Open: Companion Journal to Thrombosis and Haemostasis

Article Title: Edoxaban, a Factor Xa-Specific Direct Oral Anticoagulant, Significantly Suppresses Tumor Growth in Colorectal Cancer Colon26-Inoculated BALB/c Mice

doi: 10.1055/s-0042-1758855

Figure Lengend Snippet: Effect of edoxaban (EDX) on plasma thrombin-antithrombin complex (TAT) levels in untreated (UTR) control mice and Colon26-inoculated mice. Seven days (day 0) after inoculating mice with Colon26 cells, water or EDX (10 mg/kg/d) was orally administered daily for 21 days; citrated plasma samples were prepared from blood drawn from the heart on days -7, 0, 7, 14, and 21 of UTR mice ( n = 5) (○), water-treated group ( n = 5) (●), or EDX-treated group ( n = 5) (▪) of Colon26-inoculated mice. Plasma TAT concentration was determined by ELISA. Significant differences in plasma TAT levels between the water-treated group or EDX-treated group of Colon26-inoculated mice and the UTR mouse group are shown as ** p <0.01. Significant differences in plasma TAT levels between the water-treated group and EDX-treated group of Colon26-inoculated mice are shown as † p <0.05.

Techniques: Clinical Proteomics, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay

The proposed basic mechanism of the effect of edoxaban (EDX) on tumor growth and apoptosis mediated by factor Xa-PAR2. ( A ) In the absence of EDX, tissue factor (TF) expressed in inoculated Colon26 cells activates blood coagulation and inflammation in host mice, and generated factor Xa activates PAR2, whereas generated thrombin possibly activates PAR1/PAR4 on Colon26 cells and various stroma cells in the tumor microenvironment. PAR2 activation stimulates TF expression, STAT3-mediated IL-6, PAI-1, and MMP-2 production in peri-cancer cells, and RAS/PI3K/SyK/Ki67/cyclin D1 signaling pathways in tumor cells ( black upward arrows ). These affect tumor growth. In addition, thrombin-mediated PAR1 and PAR4 pathways induce p53 expression, leading to apoptosis, which may be regulated by the PAR2-dependent system. ( B ) EDX administration to mice suppresses factor Xa-mediated PAR2 activation, reducing PAR2 levels, followed by a decrease in plasma TF, IL-6, PAI-1, and MMP-2 levels and RAS/PI3K/SyK/Ki67/cyclin D1 signaling pathways ( white downward arrows ), leading to tumor growth suppression. Furthermore, suppression of PAR2 pathway activation by EDX may reduce the regulatory system against the PAR1/PAR4 pathway and may increase the p53-dependent apoptosis ( black upward arrows ).

Journal: TH Open: Companion Journal to Thrombosis and Haemostasis

Article Title: Edoxaban, a Factor Xa-Specific Direct Oral Anticoagulant, Significantly Suppresses Tumor Growth in Colorectal Cancer Colon26-Inoculated BALB/c Mice

doi: 10.1055/s-0042-1758855

Figure Lengend Snippet: The proposed basic mechanism of the effect of edoxaban (EDX) on tumor growth and apoptosis mediated by factor Xa-PAR2. ( A ) In the absence of EDX, tissue factor (TF) expressed in inoculated Colon26 cells activates blood coagulation and inflammation in host mice, and generated factor Xa activates PAR2, whereas generated thrombin possibly activates PAR1/PAR4 on Colon26 cells and various stroma cells in the tumor microenvironment. PAR2 activation stimulates TF expression, STAT3-mediated IL-6, PAI-1, and MMP-2 production in peri-cancer cells, and RAS/PI3K/SyK/Ki67/cyclin D1 signaling pathways in tumor cells ( black upward arrows ). These affect tumor growth. In addition, thrombin-mediated PAR1 and PAR4 pathways induce p53 expression, leading to apoptosis, which may be regulated by the PAR2-dependent system. ( B ) EDX administration to mice suppresses factor Xa-mediated PAR2 activation, reducing PAR2 levels, followed by a decrease in plasma TF, IL-6, PAI-1, and MMP-2 levels and RAS/PI3K/SyK/Ki67/cyclin D1 signaling pathways ( white downward arrows ), leading to tumor growth suppression. Furthermore, suppression of PAR2 pathway activation by EDX may reduce the regulatory system against the PAR1/PAR4 pathway and may increase the p53-dependent apoptosis ( black upward arrows ).

Techniques: Coagulation, Generated, Activation Assay, Expressing, Protein-Protein interactions, Clinical Proteomics

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